Intermediate

Part:BBa_K931005:Design

Designed by: Chris Clifford   Group: iGEM12_Nevada   (2012-09-29)


SBP - TBP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
    Illegal NotI site found at 7
    Illegal NotI site found at 350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 343
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
    Illegal NgoMIV site found at 1235
    Illegal NgoMIV site found at 1262
    Illegal AgeI site found at 425
    Illegal AgeI site found at 645
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The two genes were placed in sequence of each other with the thiamine-binding protein gene preceding the starch-binding protein gene. The linker sequence between each element is ACTAGA.



Source

The two genes come from separate organisms. The thiamine-binding protein gene comes from the genomic DNA of E. coli and the starch-binding protein gene comes from the genomic DNA of R. oryzae.


References